This is a ready-to-use membrane test based on colloidal gold particles. This test allows detection of Legionella LPS in urine samples. The test sensitivity and specificity come from monoclonal and polyclonal anti-Legionella antibodies. mouse anti-Legionella antibodies are conjugated to colloidal gold particles and dried on a conjugate absorbent pad. Each strip is sensitized with goat anti-Legionella antibodies at the T-line region and with a control antibody at the C-line region when the urine sample migrates, conjugate is rehydrated and migrates along with the sample. If L. pneumophila urinary antigens are present in the sample, a complex between the anti-L. pneumophila conjugates and the L. pneumophila antigens is formed that will be caught by the specific anti- L. pneumophila reagent coated on the stick. Results appear in 15 minutes in the form of a red line that develops on the strip.
