The immunochromatographic test utilizes a pair of Adenovirus-specific monoclonal antibodies. An extract is first prepared by suspension of the specimen in the provided extraction buffer solution. The buffer containing the extracted specimen is then added to the devices sample well. The reaction between a positive sample and the colored particle-conjugated antibody will form a complex that migrates along the membrane. An immobilized capture antibody will form a colored line at the S (specimen) area upon reacting with the colored complex. An internal control line C (control) area is built in to assure that the test has been carried out correctly. Whether the sample contains the virus or not, the solution continues to migrate to encounter another reagent (an anti-mouse IgG antibody) that binds the remaining conjugates, thereby producing a red line on the region C.
